Device

Part:BBa_K2246008:Design

Designed by: JUAN CARLOS RUEDA SILVA   Group: iGEM17_TecCEM   (2017-10-13)


WNT BSLA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 815
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 750
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BamHI restriction site as result from clonation into BBa_K2246000. A single mutation is present at at base 645 from the original BBa_K592009 sequence in order to prevent a HinCII cut site.

Source

Synthetic part.

References